July/August 2018 • www.cemag.us HOW IT WORKS 22
Problem: Keeping laboratory
equipment clean is a daunting
task. Microbes from the air,
lingering in places like cardboard boxes, or coming from
human skin can threaten the
validity of your results. At the
same time, costly cleaning procedures threaten
and productivity. To
A, for example,
decontamination procedures range from chemical
detergents to rinsing with RNase-free water and autoclaving for hours, both of which cost time and money.
Effectively decontaminating equipment at a low
operational cost is a constant battle in the lab.
Solution: Phoseon Technology’s solid-state,
deep UV LEDs save time and money, and preserve
the accuracy of your results through thorough
decontamination. Patented SLM™ technology allows
Phoseon lamps to generate orders of magnitude
higher irradiance than is currently seen in the market.
This high-irradiance light is then focused to a specific
wavelength, or combination of wavelengths, that
affect the structure of microbial contaminants.
When it comes to inactivation, irradiance matters.
Studies have shown that high irradiance 275 nm light
requires a lower total dose, less time, and more completely inactivates glass surfaces compared with lower irradiance. Complete inactivation of RNase A was
achieved after 5 minutes at high irradiance compared
with 25 minutes for the lower irradiance level.
There was also no evidence of reactivation in the
target area once the cycle was complete, confirming
inactivation was complete and irreversible. This is
possible because higher irradiance sources emit a
greater numbers of photons in the same area at the
same time. This means greater chances of disrupting
the disulfide bond in RNase A, rendering it inactive.
Thus, by reaching high irradiance levels (5W/cm2 in
Phoseon lamps compared with low m W/cm2 range
Ultraviolet Decontamination of Laboratory Equipment
seen in the market),
Phoseon lamps can
Greater, still, are
the results when
275 nm source
alone saves time.
Combining it with
a 365 nm source
saves more time and decreases the total dose
necessary to achieve inactivation.
Exposing RNase A to 365 nm light concurrently with 275 nm light reduced both the irradiance
and total dose of 275 nm wavelength necessary to achieve inactivation. Furthermore, total
inactivation of RNase A (compared with negative
control) was achieved after three minutes. Alone,
the 365 nm light source was ineffective, showing no appreciable inactivation. Working with
the 275 nm source, however, the wavelengths
act synergistically to achieve rapid inactivation.
This has significant implications for the future.
In choosing specific, targeted combinations of
light, laboratory equipment such as pipettes,
microtiter plates, glass slides, and more can be
decontaminated safely, quickly, and with less
Chemical decontamination of laboratory
equipment is a difficult task. One must successfully clean the equipment while maintaining its
integrity by carefully choosing detergents based
on equipment material. Phoseon solid-state,
deep UV LED technology eliminates this problem. Simply chose the targeted wavelength (or
wavelengths) for the material and contaminant,
and you can achieve rapid, complete inactivation, saving research time for its intended
purpose: furthering scientific knowledge.
For more information, visit www.phoseon.com.